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Image Search Results
Journal:
Article Title: The orphan nuclear receptor ROR? is a negative regulator of the inflammatory response
doi: 10.1093/embo-reports/kve007
Figure Lengend Snippet: Fig. 1. RORα is expressed in different vascular SMC types. (A and B) RT–PCR (35 cycles) analysis of RORα and GAPDH mRNA. C-PCR and C-RT are negative controls for PCR and RT, respectively; VSMC, smooth muscle cells from saphenous veins; CASMC, human coronary artery smooth muscle cells; HASMC, human aortic smooth muscle cells. (C) RT–PCR (30 cycles) analysis of RORα mRNA in SMC infected with Ad-RORα1 or Ad-GFP for 24 h. (D) Analysis of RORα protein expression in SMC infected for 24 h with or without Ad-RORα1. Immunocytochemistry experiments were performed as previously described (Chinetti et al., 1998) using a rabbit polyclonal RORα antibody raised against aa 163–225.
Article Snippet: Primary human aortic (HA) and
Techniques: Reverse Transcription Polymerase Chain Reaction, Infection, Expressing, Immunocytochemistry